Discovery and Characterization of Two Novel Salt-Tolerance Genes in Puccinellia tenuiflora
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Discovery and Characterization of Two Novel Salt-Tolerance Genes in Puccinellia tenuiflora
Puccinellia tenuiflora is a monocotyledonous halophyte that is able to survive in extreme saline soil environments at an alkaline pH range of 9-10. In this study, we transformed full-length cDNAs of P. tenuiflora into Saccharomyces cerevisiae by using the full-length cDNA over-expressing gene-hunting system to identify novel salt-tolerance genes. In all, 32 yeast clones overexpressing P. tenuif...
متن کاملIdentification and Characterization of a PutAMT1;1 Gene from Puccinellia tenuiflora
Nitrogen is one of the most important limiting factors for plant growth. However, as ammonium is readily converted into ammonia (NH3) when soil pH rises above 8.0, this activity depletes the availability of ammonium (NH4(+)) in alkaline soils, consequently preventing the growth of most plant species. The perennial wild grass Puccinellia tenuiflora is one of a few plants able to grow in soils wi...
متن کاملComparative Proteomic Analysis of Puccinellia tenuiflora Leaves under Na2CO3 Stress
Soil salt-alkalinization is a widespread environmental stress that limits crop growth and agricultural productivity. The influence of soil alkalization caused by Na(2)CO(3) on plants is more severe than that of soil salinization. Plants have evolved some unique mechanisms to cope with alkali stress; however, the plant alkaline-responsive signaling and molecular pathways are still unknown. In th...
متن کاملA Chloroplast-Localized Rubredoxin Family Protein Gene from Puccinellia tenuiflora (PutRUB) Increases NaCl and NaHCO3 Tolerance by Decreasing H2O2 Accumulation
Rubredoxin is one of the simplest iron-sulfur (Fe-S) proteins. It is found primarily in strict anaerobic bacteria and acts as a mediator of electron transfer participation in different biochemical reactions. The PutRUB gene encoding a chloroplast-localized rubredoxin family protein was screened from a yeast full-length cDNA library of Puccinellia tenuiflora under NaCl and NaHCO₃ stress. We foun...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2014
ISSN: 1422-0067
DOI: 10.3390/ijms150916469